3205 Biological Sciences II; (949) 824-6034
Jerry E. Manning, Department Chair
Faculty
Dana W. Aswad: Regulation of protein function by covalent modification
Barbara K. Burgess: Structure and function of protein-bound [FeS] and [MoFeS] clusters
Michael G. Cumsky: Mitochondrial protein import; regulation of gene expression in yeast
Rowland H. Davis: Regulation of polyamine metabolism in Neurospora crassa
Hung Fan: Molecular biology and pathogenesis of mouse and human retroviruses
Charles G. Glabe: Amyloid Aß peptide in Alzheimer's pathogenesis; gamete recognition
Gale A. Granger: Immunology and pathogenesis: Cell-mediated immunity; tumor immunology; cytokine action
Barbara A. Hamkalo: Molecular basis of differential chromatin condensation
Agnes Henschen-Edman: Protein structure, function, post-translational modification; fibrinogen
Christopher C.W. Hughes: Endothelial cells as initiators and targets of immune responses
Anthony A. James: Malaria parasite development; genetic manipulation of insect vectors
Thomas E. Lane: Molecular/immuno-pathogenic mechanisms of virus-induced demyelinating disease
Hartmut Luecke: Structure-function studies of membrane-associated proteins
Jerry E. Manning: Major surface proteins and their genes in Trypanosoma cruzi
Alexander McPherson: X-ray and atomic force microscopy analysis of protein, nucleic acid, and virus crystals; structural immunology, structural virology; microgravity research on macromolecular crystal growth
Ricardo Miledi: Neurotransmitter receptors and synaptic functions
Timothy F. Osborne: Transcriptional regulation of cholesterol biosynthesis
Thomas L. Poulos: Protein engineering and crystallography
Donald F. Senear: Interactions of proteins and DNA in transcriptional regulation
Andrea J. Tenner: Molecular basis of the enrichment of human leukocyte function
Krishna K. Tewari: Chloroplast DNA: replication and transcription
Sujata Tewari: Neuromolecular mechanisms of alcohol/drug action on the CNS
Luis P. Villarreal: Tissue-specific viral and cellular gene expression; viral vectors
Edward K. Wagner: Herpes simplex virus gene expression during productive and latent infection
Robert C. Warner: Molecular biology of nucleic acids; physical chemistry of macromolecules; mechanisms of genetic recombination
Clifford A. Woolfolk: General microbiology; enzymology
The research interests of faculty in the Department of Molecular Biology and Biochemistry include structure and synthesis of nucleic acids and proteins, regulation, virology, biochemical genetics, gene organization, nucleic acids and proteins, cell and developmental biology, molecular genetics, biomedical genetics, and immunology.
The Department offers graduate study in conjunction with the program in Molecular Biology, Genetics, and Biochemistry, which is described in a previous section. Students admitted into the combined program who select a research advisor in the Department begin following the departmental requirements for the Ph.D. at the beginning of their third year. Participation in an advanced topics seminar series and completion of at least one course per year for three years are expected of all students.
Several faculty in the Department also are members of the graduate program in Protein Engineering, which is described in a previous section.
200A-B-C Research in Molecular Biology and Biochemistry (2 to 12 per quarter) F, W, S. Individual research supervised by a particular professor. See areas of interest listed under Faculty. Prerequisite: consent of instructor.
201A-B-C Seminars in Molecular Biology and Biochemistry (2-2-2) F, W, S. Seminar, two hours. Presentation of research from department laboratories or, when pertinent, of other recent developments. Prerequisite: consent of instructor. Satisfactory/Unsatisfactory only. May be repeated for credit as topics vary.
202A-B-C Tutorial in Molecular Biology and Biochemistry (2-2-2) F, W, S. Tutorials in the area of research of a particular professor which relate current research to the literature. May be conducted as journal clubs. Prerequisite: consent of instructor. May be repeated for credit as topics vary.
203 Structure and Biosynthesis of Nucleic Acids (4) F. Lecture, three hours. The structure and properties of nucleic acids. The fundamentals of nucleic acid hybridization and recombinant DNA methodology. Replication and rearrangement of DNA. Prerequisites: Biological Sciences 98 and 99 or the equivalent and Chemistry 51A-B-C or the equivalent. (Coordinators, B. Hamkalo and D. Senear)
204 Structure and Biosynthesis of Proteins (4) F. Lecture, three hours. The structure and properties of proteins. Enzymes and their kinetic properties. Mechanisms of the biosynthesis of proteins. Prerequisites: Biological Sciences 98 and 99 or the equivalent and Chemistry 51A-B-C or the equivalent. (Coordinators, R. Bradshaw and L. Vickery)
205 Topics in Viral Gene Expression (4) W. Lecture, three hours. Primary research data on the major DNA and RNA viruses emphasizing strategies of regulation of gene expression. Utilization of viruses as molecular biological tools. Graduate-level knowledge of the biochemistry and molecular biology of macromolecules is required. Prerequisites: Molecular Biology 203 and 204 or the equivalent. (Coordinators, E. Wagner and B. Semler)
206 Regulation of Gene Expression (4) W. Lecture, three hours. Aspects of gene expression including the organization of the eukaryotic nucleus in terms of protein-nucleic acid interaction (i.e., chromatin and chromosome structure); comparisons between prokaryotic and eukaryotic gene expression, the enzymology and regulation of RNA transcription in E. Coli and other prokaryotes. Enzymology of transcription in eukaryotes. Prerequisites: Molecular Biology 203, 204, and 205. (Coordinators, R. Sandri-Goldin and C. Greer)
207 Molecular Genetics (4) S. Lecture, three hours. Recombination, genome organization, and gene expression at the molecular level, with emphasis on genetic analysis. Prerequisites: Molecular Biology 203, 204, 205, and 206. (Coordinator, R. Davis)
209 Literature in Protein Engineering (1) F, W, S. Seminar, one hour, discussion, half-hour. Students review current papers in the field of protein engineering and present the ideas contained therein to other students and faculty. May be repeated for credit. Same as Physiology 209 and Engineering CBE209.
210A-B Basic Medical Biochemistry (10-10) F, W. Lecture, ten hours. Classical and molecular biochemistry, including structure, function, and biosynthesis of macromolecules; metabolic interrelations and control mechanisms; and biochemical genetics. Application of recent advances in knowledge of molecular bases for cellular function to disease states (diagnosis, prevention, and treatment). Prerequisite: consent of instructor.
212 (4) Chromosome Dynamics in Eukaryotes (4) S every other year. Focuses on experimental approaches currently in use to investigate mechanisms by which eukaryotes carry out essential chromosomal functions. A combination of lectures and student presentations focus on these problems from the fields of genetics, cell biology, biochemistry, and molecular biology. Prerequisites: Molecular Biology and Biochemistry 203 and 204.
214 Biosynthesis of Nucleic Acids (4) F every third year beginning 1985. Lecture, three hours. Structure, function, and replication of DNA and RNA in procaryotes and eucaryotes; emphasis on current research. Prerequisite: consent of instructor. (Coordinator, S. Tewari)
217A Principles of Cancer Biology I (4) S every other year. Lecture, three hours. Oncogenes and tumor suppressor genes are studied from molecular viewpoints. Also studies their role in cancer; viral carcinogenesis. Designed for graduate students interested in cancer research. Format includes lectures and student-led discussions. Prerequisites: Molecular Biology and Biochemistry 203 and 204.
217B Principles of Cancer Biology II (4) S every other year. Lecture, three hours. Topics include cancer cell growth and metastasis, chemical carcinogenesis, and cancer genetics and epidemiology. Designed for graduate students interested in cancer research. Format includes lectures and student-led discussions. Prerequisites: Molecular Biology and Biochemistry 203 and 204.
218 Clinical Cancer (3) F of even years. Lecture, two hours. Designed to acquaint students in basic life science with clinical cancer. Restricted to graduate and postdoctoral students. May be repeated for credit. (Coordinator, H. Fan)
219 Responsible Conduct of Research (2). Each session includes a formal presentation by faculty/invited speaker followed by a discussion of case studies related to the topic under consideration. Satisfactory/Unsatisfactory only.
220 Structure and Synthesis of Biological Macromolecules Journal Club (2). Seminar, one hour. Advanced topics in macromolecular structure and synthesis as related to biological problems. Satisfactory/Unsatisfactory only. May be repeated for credit as topics vary.
221 Advanced Immunology (4) S every third year beginning 1983. Lecture, three hours; discussion, one hour. History, techniques, and concepts of humoral antibody formation and cellular immune patterns. Advanced topics in transplantation and tumor immunobiology. Prerequisite: Biological Sciences 121 or consent of instructor. (Coordinator, G. Granger)
223 Computational Molecular Biology (4) W. Lecture, one hour; laboratory, two hours. The use of computer networks, data bases, and programs in molecular biology. Students choose a protein sequence from DNA sequence data. This sequence is entered, edited, and analyzed following simulated genetic engineering. Prerequisite: consent of instructor. (Coordinator, L. Villarreal)
224 Mechanisms of Viral Transformation (4) F every third year beginning 1989. Lecture, three hours. The molecular mechanisms by which RNA and DNA tumor viruses transform cells. Emphasis on current research papers. Prerequisite: Molecular Biology and Biochemistry 205.
227 Immunology Journal Club (2) F, W, S. Seminar and discussion, one hour. Advanced topics in immunology as related to an understanding of human disease. Satisfactory/Unsatisfactory only. May be repeated for credit as topics vary.
229 Research-in-Progress Seminars (1) F, W, S. Seminar and discussion, one hour. Two half-hour presentations by graduate students and postdoctorals to the department on their current research projects. Satisfactory/Unsatisfactory only. May be taken for credit 15 times.
240 Macromolecular Structure, Function, and Interaction (4) F. Lecture, three hours; discussion, one hour. Chemistry of macromolecules; emphasis on proteins. Physical and chemical properties of proteins, forces that maintain protein structure, relationship between structure and function, interactions of proteins with ligands and other macromolecules, and experimental methods to study structure, function, and interactions. Prerequisites: Molecular Biology 203 and 204. Concurrent with Biological Sciences 140. (Coordinators: D. Senear and T. Poulos)
250 Recombinant DNA Technology (4). F, W, S. Laboratory, four hours. Individual training in major techniques of recombinant DNA, including extraction and purification of nucleic acids, cloning and subcloning, DNA sequencing, nucleic acid hybridization, and associated procedures. Student must demonstrate accurate documentation of data with laboratory notebook detailing experience.
251 Protein Isolation and Characterization (4) F, W, S. Individual training in major techniques of handling proteins, including isolation, various purification procedures, characterization, and tests for biological or catalytic activity. Student must demonstrate accurate documentation of data with laboratory notebook detailing experience.
252 Animal and Microbial Cell Culture (4) F, W, S. Individual training in techniques of animal and microbial cell culture, particularly as required in immunological and virological laboratories. Student must demonstrate accurate documentation of data with laboratory notebook detailing experience.
254 Protein Crystallography (3) S of even years. Lecture, three hours. Introduces students to the theory and practice of macromolecular crystallography. Covers all aspects, including protein crystallization, space groups, phasing methods, electron density map interpretation, refinement and preparation of results for publication. Corequisite: calculus. Prerequisite: consent of instructor. Same as Physiology and Biophysics 211.
280 Advanced Topics in Biochemistry and Molecular Biology (3) F. Lecture, five hours. Selected topics in specified areas of concentration, e.g., nucleic acids, protein biochemistry, genetic expression, biochemical genetics. Specific topics announced in advance. Prerequisites: Biological Sciences 106 and 107 and consent of instructor. Normally taken with Molecular Biology and Biochemistry 205A. Open to advanced undergraduates.
290A-B-C Colloquium in Molecular Biology and Biochemistry (2-2-2) F, W, S. Colloquium, one and one-half hours. Contemporary research problems in molecular biology and biochemistry. Invited speakers present research and/or review topics. Satisfactory/Unsatisfactory only. May be repeated for credit.
399 University Teaching (4-4-4) F, W, S. Limited to Teaching Assistants.
